The effects of fullerenol nanoparticles on erythrocyte deformability in sevoflurane applied rats

  • Volkan Sivgin Department of Anesthesiology and Reanimation, Faculty of Medicine, Gazi University, Ankara, Turkey.
  • Aysegul Kucuk Department of Physiology, Faculty of Medicine, Kütahya Health Sciences Universty, Kütahya, Turkey.
  • Faruk Metin Comu Department of Physiology, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey.
  • Ayse Gulfem Yalcin Department of Anesthesiology and Reanimation, Faculty of Medicine, Gazi University, Ankara, Turkey.
  • Mustafa Arslan Department of Anesthesiology and Reanimation, Faculty of Medicine, Gazi University, Ankara, Turkey.
DOI: https://doi.org/10.35975/apic.v25i5.1619
Keywords: Animals, Rats, Sevoflurane, Fullerenol, Fullerenes* / pharmacology, Nanoparticles, Oxidative Stress, Erythrocyte deformability

Abstract

Background & objective: Oxidative damage causing alterations in erythrocyte deformability due to anesthesia might be one of the factors responsible for the deterioration of the tissue and organ perfusion. The antioxidant properties of fullerenol nanoparticles, used in medical field together with the developing technology, have been shown in the literature. We investigated the effects of fullerenol nanoparticles, used before sevoflurane anesthesia, on the erythrocyte deformability in the rats.

Methodology: Twenty-four male Wistar Albino rats were used in this study and randomly divided into four groups, six in each group; Group C (Control Group), Group S (Group Sevoflurane), Group F (Group Fullerenol), Group FS (Group Fullerenol-Sevoflurane). Fullerenol was given to the Group F and Group FS at a dose of 100 mg/kg. Sevoflurane was administered to rats in the Groups S and FS at 3% concentration. Erytrocytes deformability was measured by the constant-current filtrometre system and the deformability index was interpreted. All the data were processed by variance analysis in SPSS 22.0 for Windows statistical software. Variance analysis and Kruskal-Wallis test were used to evaluate the data. Mann–Whitney U test with Bonferroni correction were used to evaluate the variables with significance.

Results: Relative resistance increased in all groups due to sevoflurane administration (p < 0.0001). The erythrocyte deformability index was significantly higher in the sevoflurane group than in the control and fullerenol groups (p < 0.0001, p = 0.002, respectively). Fullerenol administration, before 30 min of sevoflurane treatment, was found to decrease erythrocyte deformability index significantly compared to sevoflurane group (p = 0.017).

Conclusion: Administring 100 mg/kg fullerenol nanoparticles intraperitoneally 30 min before sevoflurane reduces erythrocyte deformability.

Key words: Animals; Rats; Sevoflurane; Fullerenol; Fullerenes* / pharmacology; Nanoparticles; Oxidative Stress; Erythrocyte deformability

Citation: Sivgin V, Kucuk A , Comu FM , Yalcin SG, Arslan M. The effects of fullerenol nanoparticles on erythrocyte deformability in sevoflurane applied rats. Anaesth. pain intensive care 2021;25(5):602–606

DOI: 10.35975/apic.v25i5.1619

Received: March 28, 2021, Reviewed: July 9, 2021, Accepted: August 20, 2021

Published
09-30-2021
How to Cite
Sivgin, V., Kucuk, A., Comu, F., Yalcin, A., & Arslan, M. (2021). The effects of fullerenol nanoparticles on erythrocyte deformability in sevoflurane applied rats. Anaesthesia, Pain & Intensive Care, 25(5), 602–606. https://doi.org/10.35975/apic.v25i5.1619
Issue
Vol 25 No 5 (2021): September-October
Section
ORIGINAL RESEARCH